中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (52): 8420-8425.doi: 10.3969/j.issn.2095-4344.2014.52.011

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

聚乙烯界面结核分枝杆菌黏附力及生物膜的形成

熊庆广1，王永清1，赵志辉1，毕红宾2，孙晋保1，张庆杰2，郝晓辉1，李 毅1   

  1. 1天津医科大学第四中心临床学院，天津市 300140；2天津中医药大学，天津市 300139
  • 修回日期:2014-11-12 出版日期:2014-12-17 发布日期:2014-12-17
  • 通讯作者: 王永清，教授，主任医师，硕士生导师，博士生导师，天津医科大学第四中心临床学院，天津市 300140
  • 作者简介:熊庆广，男，1988年生，江西省南昌市人，汉族，天津医科大学在读硕士，主要从事骨与关节疾病的研究。
  • 基金资助:

    天津市卫生局科技基金项目(2013KY05)

Adhesion ability and biofilm formation of Mycobacterium tuberculosis in the interface of polyethylene

Xiong Qing-guang1, Wang Yong-qing1, Zhao Zhi-hui1, Bi Hong-bin2, Sun Jin-bao1, Zhang Qing-jie2, Hao Xiao-hui1, Li Yi1   

  1. 1Department of Orthopedics, the Fourth Central Clinical College, Tianjin Medical University, Tianjin 300140, China; 2Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
  • Revised:2014-11-12 Online:2014-12-17 Published:2014-12-17
  • Contact: Wang Yong-qing, Professor, Chief physician, Master’s supervisor, Doctoral supervisor, Department of Orthopedics, the Fourth Central Clinical College, Tianjin Medical University, Tianjin 300140, China
  • About author:Xiong Qing-guang, Studying for master’s degree, Department of Orthopedics, the Fourth Central Clinical College, Tianjin Medical University, Tianjin 300140, China
  • Supported by:

    Science and Technology Foundation of Tianjin Bureau of Public Health, No. 2013KY05

PDF

484

被引次数

1

摘要：

背景：研究证实结核杆菌对钴铬钼、钛合金的黏附力低，且不形成生物膜，但其对聚乙烯的黏附力及生物膜形成情况未见报道。

目的：观察利福平干预对聚乙烯界面结核杆菌黏附力及生物膜形成的影响。
方法：将聚乙烯和钴铬钼实验材料在无菌条件下分别置于Middlebrook 7H9液体培养基中，与结核杆菌共同培养2周，随机取出2块后，再加入1 mg/L的利福平溶2 mL，继续培养2周后，用激光共聚焦显微镜观测利福平干预前后材料界面单位面积菌落数及菌落厚度，扫描电镜观察利福平干预前后材料界面细菌生物膜结构。
结果与结论：利福平干预前，聚乙烯界面单位面积菌落数及菌落厚度均大于钴铬钼组(P < 0.05)；利福平干预后，聚乙烯界面单位面积菌落数及菌落厚度较干预前明显减少(P < 0.05)。利福平干预前，聚乙烯界面可见结核杆菌黏附，形成似“雪花状”、“云雾状”，可见生物膜结构；钴铬钼界面可见散在结核杆菌菌落，未见生物膜结构形成。利福平干预后，聚乙烯界面黏附菌落数减少，部分结核杆菌出现干瘪、裂解，生物膜有不同程度的破坏；钴铬钼界面未见菌落形成，偶见单个结核杆菌黏附，未见生物膜形成。表明结核杆菌可在聚乙烯界面形成生物膜，但利福平可抑制甚至破坏结核生物膜。

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 材料相容性, 聚乙烯, 钴铬钼, 结核分枝杆菌, 利福平, 关节结核, 生物膜

Abstract:

BACKGROUND: Studies have confirmed that there is no biofilm formation and lower adhesion ability of Mycobacterium tuberculosis in the interface of vitallium, but it has not been reported about biofilm formation and adhesion ability in the polyethylene.

OBJECTIVE: To observe the adhesion ability and biofilm formation of Mycobacterium tuberculosis on the polyethylene before and after rifampicin intervention.
METHODS: Under sterile conditions, polyethylene and vitallium samples were respectively placed in Middlebrook 7H9 culture medium to be co-cultured with Mycobacterium tuberculosis for 2 weeks. Then, two pieces of samples were taken randomly and cultured in 1 mg/L rifampicin (2 mL) for 2 weeks. The number of colonies and colony thickness in the unit area in the material interface were measured by confocal laser scanning microscope, and the structure of biofilms was observed by scanning electron microscope before and after rifampicin intervention.
RESULTS AND CONCLUSION: The colony thickness and the number of colonies in the unit area was significantly more in the polyethylene group than in the vitallium group before intervention (P < 0.05), but after intervention, these two indexes were reduced significantly in the polyethylene group (P < 0.05). Before rifampicin intervention, Mycobacterium tuberculosis was adherent to the polyethylene interface in a snowflake-like or nebulous shape, and biofilm structure was visible; in the vitallium interface, there were scattered colonies of Mycobacterium tuberculosis, but no biofilm formation. After rifampicin intervention, the number of colonies adhered in the polyethylene interface was reduced, Mycobacterium tuberculosis appeared to be shriveled and cleaved partially, and the biofilms exhibited varying degrees of damage; in the vitallium interface, there were no colonies, but single Mycobacterium tuberculosis was occasionally adherent to the polyethylene interface, and there was no biofilm formation. These findings indicate that Mycobacterium tuberculosis can form a biofilm in the polyethylene interface, but rifampin can inhibit and even destroy the biofilm of Mycobacterium tuberculosis.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: polyethylene, vitallium, Mycobacterium tuberculosis, rifampin

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